Since you have already recorded the file, you can use Digital Media Converter by DeskShare to split the file. You can also convert the file to almost all popular formats. For more information on Digital Media Converter, visit:
https://www.deskshare.com/audio-video-converter.aspx
Another option is that you can start using My Screen Recorder Pro for any recordings you do in the future. The Pro version can automatically segment the file to pre-defined sizes. The segmenting option is available from Tools ] Settings ] File segments.